Journal: Diseases

Article Title: Cyclosporine A Causes Gingival Overgrowth by Promoting Entry into the S Phase at the G1/S Cell Cycle Checkpoint in Gingival Fibroblasts Exposed to Lipopolysaccharide

doi: 10.3390/diseases12120322

Figure Lengend Snippet: The proliferation of gingival fibroblasts stimulated with or without LPS or cyclosporine A in the culture with D-MEM plus serum. After semi-confluent cells were cultured in D-MEM containing 1% serum for 24 h, cells were treated with or without 200 ng/mL of cyclosporine A or 1 μg/mL of LPS in D-MEM containing 5% serum for 24 or 48 h. The total number of cells was measured with Trypan Blue dye exclusion using a blood corpuscle counting chamber and an optical microscope. The fold change from the negative control was calculated. Data are presented as means ± SEM. Welch’s t -test with Bonferroni’s correction was used for statistics. * p < 0.05 indicates a statistically significant difference. n = 4. Cs A, cyclosporine A.

Article Snippet: We counted the total number of cells to evaluate cell proliferation with Trypan Blue dye exclusion using a blood corpuscle counting chamber (Burker-Turk deep 1/10 mm, ERMA Inc., Tokyo, Japan) and observed the cellular morphology using an optical microscope (Nikon TE300 Inverted Tissue Culture Microscope, Tokyo, Japan; magnification, 40×).

Techniques: Cell Culture, Microscopy, Negative Control